286 Evaluation of Empore™ C18 Spin Column Ability to Increase Proteome Coverage via High pH Fractionation
Sample preparation is paramount to produce high quality LC-MS proteomic data. Large, bottom-up proteomic coverage from a single sample can be difficult to achieve due to the depth and complexity of a peptide population. Instrument systems for sample fractionation are expensive, require time, and regular maintenance for reliable results. Here we present a quick and affordable option for larger sample frationation, the Empore™ C18 spin column. We showcase improved proteome coverage of E. coli peptides sample by preparation via desalting combined with high pH reverse phase fractionation.
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